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hrptec tert1 cells  (ATCC)


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    ATCC hrptec tert1 cells
    Effect of uranium on apoptosis and necrosis for hRPTEC <t>TERT1</t> cells. Cells were treated with different concentrations of uranium for 48 h. Next, cell apoptosis was measured by caspase 3/7 luminescence assay and cell necrosis was measured by LDH luminescence assay. Data were expressed as a percentage of the positive control, treatment with staurosporine 10 μM over 24 h for apoptosis induction, and treatment with triton 1% for 15 min for necrosis induction. Data were expressed as mean ± SEM where n = 8 per condition. Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).
    Hrptec Tert1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rptec+tert1+cells/RPTEC%2FTERT1/pmc13276292-66-1-4
    Average 95 stars, based on 196 article reviews
    hrptec tert1 cells - by Bioz Stars, 2026-09
    95/100 stars

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    1) Product Images from "In Vitro hRPTEC TERT1 Model for Uranium‐Induced Nephrotoxicity Pathway Study"

    Article Title: In Vitro hRPTEC TERT1 Model for Uranium‐Induced Nephrotoxicity Pathway Study

    Journal: Journal of Toxicology

    doi: 10.1155/jt/6692188

    Effect of uranium on apoptosis and necrosis for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. Next, cell apoptosis was measured by caspase 3/7 luminescence assay and cell necrosis was measured by LDH luminescence assay. Data were expressed as a percentage of the positive control, treatment with staurosporine 10 μM over 24 h for apoptosis induction, and treatment with triton 1% for 15 min for necrosis induction. Data were expressed as mean ± SEM where n = 8 per condition. Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).
    Figure Legend Snippet: Effect of uranium on apoptosis and necrosis for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. Next, cell apoptosis was measured by caspase 3/7 luminescence assay and cell necrosis was measured by LDH luminescence assay. Data were expressed as a percentage of the positive control, treatment with staurosporine 10 μM over 24 h for apoptosis induction, and treatment with triton 1% for 15 min for necrosis induction. Data were expressed as mean ± SEM where n = 8 per condition. Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).

    Techniques Used: Luminescence Assay, Positive Control

    Effect of uranium on apoptosis or survival pathway for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. (a) Screening of gene expression implicated in apoptosis or survival pathway. Data were expressed as mean ± SEM ( n = 8 for 0, 100 and 300 μM; n = 5 for 500 μM). (b) Proteome profiler array on apoptosis or survival pathway. Results are expressed as a chemiluminescence signal ratio (treated to untreated cells) in the form of a heat map ( n = 1 per condition). Asterisk represents a significant difference between U (VI) treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).
    Figure Legend Snippet: Effect of uranium on apoptosis or survival pathway for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. (a) Screening of gene expression implicated in apoptosis or survival pathway. Data were expressed as mean ± SEM ( n = 8 for 0, 100 and 300 μM; n = 5 for 500 μM). (b) Proteome profiler array on apoptosis or survival pathway. Results are expressed as a chemiluminescence signal ratio (treated to untreated cells) in the form of a heat map ( n = 1 per condition). Asterisk represents a significant difference between U (VI) treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).

    Techniques Used: Gene Expression

    Effect of uranium on nephrotoxicity biomarkers for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. (a) Gene expression of nephrotoxicity biomarkers. Data were expressed as mean ± SEM ( n = 3 per condition). (b) Proteome profiler array on nephrotoxicity. Results are expressed as a ratio of untreated to treated cells at different concentrations in the form of a heat map ( n = 1 per condition). (c) KIM1 protein assay by Milliplex (LOQ 0.05 ng.mL −1 ). Data were expressed as mean ± SD ( n = 3 per condition). (d) Collagen IV protein assay by Milliplex (LOQ 0.5 ng.mL −1 ). Data were expressed as mean ± SD ( n = 3 per condition). Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001).
    Figure Legend Snippet: Effect of uranium on nephrotoxicity biomarkers for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. (a) Gene expression of nephrotoxicity biomarkers. Data were expressed as mean ± SEM ( n = 3 per condition). (b) Proteome profiler array on nephrotoxicity. Results are expressed as a ratio of untreated to treated cells at different concentrations in the form of a heat map ( n = 1 per condition). (c) KIM1 protein assay by Milliplex (LOQ 0.05 ng.mL −1 ). Data were expressed as mean ± SD ( n = 3 per condition). (d) Collagen IV protein assay by Milliplex (LOQ 0.5 ng.mL −1 ). Data were expressed as mean ± SD ( n = 3 per condition). Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001).

    Techniques Used: Gene Expression

    Effect of uranium on oxidative stress for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium (U (VI)) for 48 h. (a) Gene expression of antioxidant enzymes. Results are expressed as a ratio to the expression of the housekeeping gene GAPDH. Data were expressed as mean ± SEM ( n = 8 for 0, 100, and 300 μM; n = 5 for 500 μM). (b) Gene expression of targets implicated in the oxidative stress pathway. Results are expressed as a ratio to the expression of the housekeeping gene GAPDH. Data were expressed as mean ± SEM ( n = 3 per condition). (c) Proteome profiler array on oxidative stress pathway. Results are expressed as chemiluminescence signal ratio (U (VI) treated to untreated cells) in the form of a heat map ( n = 1 per condition). (d) Kinetics of reactive oxygen species production after exposure to U (VI) for 48 h ( n = 3 per condition). Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U(VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).
    Figure Legend Snippet: Effect of uranium on oxidative stress for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium (U (VI)) for 48 h. (a) Gene expression of antioxidant enzymes. Results are expressed as a ratio to the expression of the housekeeping gene GAPDH. Data were expressed as mean ± SEM ( n = 8 for 0, 100, and 300 μM; n = 5 for 500 μM). (b) Gene expression of targets implicated in the oxidative stress pathway. Results are expressed as a ratio to the expression of the housekeeping gene GAPDH. Data were expressed as mean ± SEM ( n = 3 per condition). (c) Proteome profiler array on oxidative stress pathway. Results are expressed as chemiluminescence signal ratio (U (VI) treated to untreated cells) in the form of a heat map ( n = 1 per condition). (d) Kinetics of reactive oxygen species production after exposure to U (VI) for 48 h ( n = 3 per condition). Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U(VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).

    Techniques Used: Gene Expression, Expressing

    Effect of uranium on inflammation for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. (a) Screening of gene expression implicated in the inflammation pathway. Data were expressed as mean ± SEM ( n = 3 per condition). (b) Proteome profiler array on inflammation pathway. Results are expressed as a ratio of untreated to treated cells at different concentrations in the form of a heat map ( n = 1 per condition). (c) TNFα protein assay by Milliplex. Data were expressed as mean ± SD ( n = 3 per condition). (d) IL‐6 protein assay by Milliplex. Data were expressed as mean ± SD ( n = 3 per condition). (e) IL‐18 protein assay by Milliplex. Data were expressed as mean ± SD ( n = 3 per condition). Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).
    Figure Legend Snippet: Effect of uranium on inflammation for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. (a) Screening of gene expression implicated in the inflammation pathway. Data were expressed as mean ± SEM ( n = 3 per condition). (b) Proteome profiler array on inflammation pathway. Results are expressed as a ratio of untreated to treated cells at different concentrations in the form of a heat map ( n = 1 per condition). (c) TNFα protein assay by Milliplex. Data were expressed as mean ± SD ( n = 3 per condition). (d) IL‐6 protein assay by Milliplex. Data were expressed as mean ± SD ( n = 3 per condition). (e) IL‐18 protein assay by Milliplex. Data were expressed as mean ± SD ( n = 3 per condition). Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).

    Techniques Used: Gene Expression

    Related Articles

    Cell Culture:

    Article Title: A VPS33B CRISPR knockout study: In vitro evidence of an adhesion defect.
    Article Snippet: .. RPTEC-TERT1 cells (ATCC, #CRL-4031TM) were cultured in DMEM:F12 Medium (ATCC® 30–2006TM), supplemented with hTERT RPTEC Growth Kit (ATCC® ACS-4007TM) (1% Supplement A and 1.6% Supplement B), 2% FBS, and 0.1 mg/mL GeneticinTM Selective Antibiotic (G418 Sulfate) (Thermo Fisher, #10131035). ..

    Article Title: Noncytotoxic polymyxin derivatives enhance antibiotic action against multidrug-resistant Gram-negative bacteria.
    Article Snippet: .. RPTEC/TERT1 cells were cultured in T75 flasks with Dulbecco’s modi fied Eagle’s medium:F12 supplemented with the ATCC (United States) human telo merase reverse transcriptase immortalized RPTEC growth kit, G418 (0.1 mg/mL final Month XXXX Volume 0 Issue 0 10.1128/aac.00712-2516 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/a ac o n 27 S ep te m be r 20 25 b y 2a 09 :b ac 5: d5 7f :1 eb ::3 1: ea . concentration), and 2% FBS. ..

    Article Title: The LINC00920/miR-6834-3p/CPN1 Axis Modulates Immune Dysregulation in Sepsis-Associated Acute Kidney Injury and Serves as a Diagnostic Biomarker.
    Article Snippet: Sepsis-associated acute kidney injury (SA-AKI) is a serious complication with high mortality and lacks effective diagnostic and treatment strategies.. This study aims to investigate the role of the LINC00920/miR-6834-3p/CPN1 axis in SA-AKI and evaluate its diagnostic value.. Clinical serum samples and in vitro cell models were used to explore the role of the LINC00920/miR-6834-3p/CPN1 axis in SA-AKI.

    Article Title: Mitochondrial toxicity of emerging disinfection byproduct 2,6-dichloro-1,4- benzoquinone in renal proximal tubule cells: Oxidative phosphorylation impairment and metabolic reprogramming.
    Article Snippet: 2,6-Dichloro-1,4-benzoquinone (DCBQ), an emerging aromatic disinfection byproduct (DBP) frequently detected in drinking water, has raised increasing concern regarding its potential renal toxicity.. However, its toxic effects at environmentally relevant concentrations and the underlying mechanisms remain unclear.. In this study, human renal proximal tubule epithelial cells (RPTEC/TERT1) were exposed to intrarenal-equivalent levels of DCBQ (10, 100, and 1000 μg/L) to investigate mitochondrial toxicity under a worst-case exposure scenario.

    Article Title: A VPS33B CRISPR knockout study: In vitro evidence of an adhesion defect
    Article Snippet: .. RPTEC-TERT1 cells (ATCC, #CRL-4031TM) were cultured in DMEM:F12 Medium (ATCC® 30–2006TM), supplemented with hTERT RPTEC Growth Kit (ATCC® ACS-4007TM) (1% Supplement A and 1.6% Supplement B), 2% FBS, and 0.1 mg/mL GeneticinTM Selective Antibiotic (G418 Sulfate) (Thermo Fisher, #10131035). ..

    Article Title: Noncytotoxic polymyxin derivatives enhance antibiotic action against multidrug-resistant Gram-negative bacteria
    Article Snippet: .. RPTEC/TERT1 cells were cultured in T75 flasks with Dulbecco’s modified Eagle’s medium:F12 supplemented with the ATCC (United States) human telomerase reverse transcriptase immortalized RPTEC growth kit, G418 (0.1 mg/mL final concentration), and 2% FBS. ..

    Reverse Transcription:

    Article Title: Noncytotoxic polymyxin derivatives enhance antibiotic action against multidrug-resistant Gram-negative bacteria.
    Article Snippet: .. RPTEC/TERT1 cells were cultured in T75 flasks with Dulbecco’s modi fied Eagle’s medium:F12 supplemented with the ATCC (United States) human telo merase reverse transcriptase immortalized RPTEC growth kit, G418 (0.1 mg/mL final Month XXXX Volume 0 Issue 0 10.1128/aac.00712-2516 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/a ac o n 27 S ep te m be r 20 25 b y 2a 09 :b ac 5: d5 7f :1 eb ::3 1: ea . concentration), and 2% FBS. ..

    Article Title: Noncytotoxic polymyxin derivatives enhance antibiotic action against multidrug-resistant Gram-negative bacteria
    Article Snippet: .. RPTEC/TERT1 cells were cultured in T75 flasks with Dulbecco’s modified Eagle’s medium:F12 supplemented with the ATCC (United States) human telomerase reverse transcriptase immortalized RPTEC growth kit, G418 (0.1 mg/mL final concentration), and 2% FBS. ..

    Concentration Assay:

    Article Title: Noncytotoxic polymyxin derivatives enhance antibiotic action against multidrug-resistant Gram-negative bacteria.
    Article Snippet: .. RPTEC/TERT1 cells were cultured in T75 flasks with Dulbecco’s modi fied Eagle’s medium:F12 supplemented with the ATCC (United States) human telo merase reverse transcriptase immortalized RPTEC growth kit, G418 (0.1 mg/mL final Month XXXX Volume 0 Issue 0 10.1128/aac.00712-2516 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/a ac o n 27 S ep te m be r 20 25 b y 2a 09 :b ac 5: d5 7f :1 eb ::3 1: ea . concentration), and 2% FBS. ..

    Article Title: Noncytotoxic polymyxin derivatives enhance antibiotic action against multidrug-resistant Gram-negative bacteria
    Article Snippet: .. RPTEC/TERT1 cells were cultured in T75 flasks with Dulbecco’s modified Eagle’s medium:F12 supplemented with the ATCC (United States) human telomerase reverse transcriptase immortalized RPTEC growth kit, G418 (0.1 mg/mL final concentration), and 2% FBS. ..

    Modification:

    Article Title: Mitochondrial toxicity of emerging disinfection byproduct 2,6-dichloro-1,4- benzoquinone in renal proximal tubule cells: Oxidative phosphorylation impairment and metabolic reprogramming.
    Article Snippet: 2,6-Dichloro-1,4-benzoquinone (DCBQ), an emerging aromatic disinfection byproduct (DBP) frequently detected in drinking water, has raised increasing concern regarding its potential renal toxicity.. However, its toxic effects at environmentally relevant concentrations and the underlying mechanisms remain unclear.. In this study, human renal proximal tubule epithelial cells (RPTEC/TERT1) were exposed to intrarenal-equivalent levels of DCBQ (10, 100, and 1000 μg/L) to investigate mitochondrial toxicity under a worst-case exposure scenario.

    Article Title: Noncytotoxic polymyxin derivatives enhance antibiotic action against multidrug-resistant Gram-negative bacteria
    Article Snippet: .. RPTEC/TERT1 cells were cultured in T75 flasks with Dulbecco’s modified Eagle’s medium:F12 supplemented with the ATCC (United States) human telomerase reverse transcriptase immortalized RPTEC growth kit, G418 (0.1 mg/mL final concentration), and 2% FBS. ..



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    Effect of uranium on apoptosis and necrosis for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. Next, cell apoptosis was measured by caspase 3/7 luminescence assay and cell necrosis was measured by LDH luminescence assay. Data were expressed as a percentage of the positive control, treatment with staurosporine 10 μM over 24 h for apoptosis induction, and treatment with triton 1% for 15 min for necrosis induction. Data were expressed as mean ± SEM where n = 8 per condition. Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).

    Journal: Journal of Toxicology

    Article Title: In Vitro hRPTEC TERT1 Model for Uranium‐Induced Nephrotoxicity Pathway Study

    doi: 10.1155/jt/6692188

    Figure Lengend Snippet: Effect of uranium on apoptosis and necrosis for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. Next, cell apoptosis was measured by caspase 3/7 luminescence assay and cell necrosis was measured by LDH luminescence assay. Data were expressed as a percentage of the positive control, treatment with staurosporine 10 μM over 24 h for apoptosis induction, and treatment with triton 1% for 15 min for necrosis induction. Data were expressed as mean ± SEM where n = 8 per condition. Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).

    Article Snippet: The hRPTEC TERT1 cells (ATCC, CRL‐4031) were maintained in a DMEM/F12 medium (Cytivia), supplemented with 10 ng/mL of hEGF, 0.1 mg/mL of G418, 1% ITSG, 3.5 μg/mL of ascorbic acid, and 25 ng/mL of prostaglandin E1 (ATCC).

    Techniques: Luminescence Assay, Positive Control

    Effect of uranium on apoptosis or survival pathway for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. (a) Screening of gene expression implicated in apoptosis or survival pathway. Data were expressed as mean ± SEM ( n = 8 for 0, 100 and 300 μM; n = 5 for 500 μM). (b) Proteome profiler array on apoptosis or survival pathway. Results are expressed as a chemiluminescence signal ratio (treated to untreated cells) in the form of a heat map ( n = 1 per condition). Asterisk represents a significant difference between U (VI) treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).

    Journal: Journal of Toxicology

    Article Title: In Vitro hRPTEC TERT1 Model for Uranium‐Induced Nephrotoxicity Pathway Study

    doi: 10.1155/jt/6692188

    Figure Lengend Snippet: Effect of uranium on apoptosis or survival pathway for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. (a) Screening of gene expression implicated in apoptosis or survival pathway. Data were expressed as mean ± SEM ( n = 8 for 0, 100 and 300 μM; n = 5 for 500 μM). (b) Proteome profiler array on apoptosis or survival pathway. Results are expressed as a chemiluminescence signal ratio (treated to untreated cells) in the form of a heat map ( n = 1 per condition). Asterisk represents a significant difference between U (VI) treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).

    Article Snippet: The hRPTEC TERT1 cells (ATCC, CRL‐4031) were maintained in a DMEM/F12 medium (Cytivia), supplemented with 10 ng/mL of hEGF, 0.1 mg/mL of G418, 1% ITSG, 3.5 μg/mL of ascorbic acid, and 25 ng/mL of prostaglandin E1 (ATCC).

    Techniques: Gene Expression

    Effect of uranium on nephrotoxicity biomarkers for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. (a) Gene expression of nephrotoxicity biomarkers. Data were expressed as mean ± SEM ( n = 3 per condition). (b) Proteome profiler array on nephrotoxicity. Results are expressed as a ratio of untreated to treated cells at different concentrations in the form of a heat map ( n = 1 per condition). (c) KIM1 protein assay by Milliplex (LOQ 0.05 ng.mL −1 ). Data were expressed as mean ± SD ( n = 3 per condition). (d) Collagen IV protein assay by Milliplex (LOQ 0.5 ng.mL −1 ). Data were expressed as mean ± SD ( n = 3 per condition). Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001).

    Journal: Journal of Toxicology

    Article Title: In Vitro hRPTEC TERT1 Model for Uranium‐Induced Nephrotoxicity Pathway Study

    doi: 10.1155/jt/6692188

    Figure Lengend Snippet: Effect of uranium on nephrotoxicity biomarkers for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. (a) Gene expression of nephrotoxicity biomarkers. Data were expressed as mean ± SEM ( n = 3 per condition). (b) Proteome profiler array on nephrotoxicity. Results are expressed as a ratio of untreated to treated cells at different concentrations in the form of a heat map ( n = 1 per condition). (c) KIM1 protein assay by Milliplex (LOQ 0.05 ng.mL −1 ). Data were expressed as mean ± SD ( n = 3 per condition). (d) Collagen IV protein assay by Milliplex (LOQ 0.5 ng.mL −1 ). Data were expressed as mean ± SD ( n = 3 per condition). Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001).

    Article Snippet: The hRPTEC TERT1 cells (ATCC, CRL‐4031) were maintained in a DMEM/F12 medium (Cytivia), supplemented with 10 ng/mL of hEGF, 0.1 mg/mL of G418, 1% ITSG, 3.5 μg/mL of ascorbic acid, and 25 ng/mL of prostaglandin E1 (ATCC).

    Techniques: Gene Expression

    Effect of uranium on oxidative stress for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium (U (VI)) for 48 h. (a) Gene expression of antioxidant enzymes. Results are expressed as a ratio to the expression of the housekeeping gene GAPDH. Data were expressed as mean ± SEM ( n = 8 for 0, 100, and 300 μM; n = 5 for 500 μM). (b) Gene expression of targets implicated in the oxidative stress pathway. Results are expressed as a ratio to the expression of the housekeeping gene GAPDH. Data were expressed as mean ± SEM ( n = 3 per condition). (c) Proteome profiler array on oxidative stress pathway. Results are expressed as chemiluminescence signal ratio (U (VI) treated to untreated cells) in the form of a heat map ( n = 1 per condition). (d) Kinetics of reactive oxygen species production after exposure to U (VI) for 48 h ( n = 3 per condition). Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U(VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).

    Journal: Journal of Toxicology

    Article Title: In Vitro hRPTEC TERT1 Model for Uranium‐Induced Nephrotoxicity Pathway Study

    doi: 10.1155/jt/6692188

    Figure Lengend Snippet: Effect of uranium on oxidative stress for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium (U (VI)) for 48 h. (a) Gene expression of antioxidant enzymes. Results are expressed as a ratio to the expression of the housekeeping gene GAPDH. Data were expressed as mean ± SEM ( n = 8 for 0, 100, and 300 μM; n = 5 for 500 μM). (b) Gene expression of targets implicated in the oxidative stress pathway. Results are expressed as a ratio to the expression of the housekeeping gene GAPDH. Data were expressed as mean ± SEM ( n = 3 per condition). (c) Proteome profiler array on oxidative stress pathway. Results are expressed as chemiluminescence signal ratio (U (VI) treated to untreated cells) in the form of a heat map ( n = 1 per condition). (d) Kinetics of reactive oxygen species production after exposure to U (VI) for 48 h ( n = 3 per condition). Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U(VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).

    Article Snippet: The hRPTEC TERT1 cells (ATCC, CRL‐4031) were maintained in a DMEM/F12 medium (Cytivia), supplemented with 10 ng/mL of hEGF, 0.1 mg/mL of G418, 1% ITSG, 3.5 μg/mL of ascorbic acid, and 25 ng/mL of prostaglandin E1 (ATCC).

    Techniques: Gene Expression, Expressing

    Effect of uranium on inflammation for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. (a) Screening of gene expression implicated in the inflammation pathway. Data were expressed as mean ± SEM ( n = 3 per condition). (b) Proteome profiler array on inflammation pathway. Results are expressed as a ratio of untreated to treated cells at different concentrations in the form of a heat map ( n = 1 per condition). (c) TNFα protein assay by Milliplex. Data were expressed as mean ± SD ( n = 3 per condition). (d) IL‐6 protein assay by Milliplex. Data were expressed as mean ± SD ( n = 3 per condition). (e) IL‐18 protein assay by Milliplex. Data were expressed as mean ± SD ( n = 3 per condition). Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).

    Journal: Journal of Toxicology

    Article Title: In Vitro hRPTEC TERT1 Model for Uranium‐Induced Nephrotoxicity Pathway Study

    doi: 10.1155/jt/6692188

    Figure Lengend Snippet: Effect of uranium on inflammation for hRPTEC TERT1 cells. Cells were treated with different concentrations of uranium for 48 h. (a) Screening of gene expression implicated in the inflammation pathway. Data were expressed as mean ± SEM ( n = 3 per condition). (b) Proteome profiler array on inflammation pathway. Results are expressed as a ratio of untreated to treated cells at different concentrations in the form of a heat map ( n = 1 per condition). (c) TNFα protein assay by Milliplex. Data were expressed as mean ± SD ( n = 3 per condition). (d) IL‐6 protein assay by Milliplex. Data were expressed as mean ± SD ( n = 3 per condition). (e) IL‐18 protein assay by Milliplex. Data were expressed as mean ± SD ( n = 3 per condition). Asterisk represents a significant difference between U (VI)‐treated and untreated cells (two‐way ANOVA ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001); sharp sign represents a significant difference between U (VI)‐treated concentrations (two‐way ANOVA # p < 0.05; ## p < 0.01; ### p < 0.001).

    Article Snippet: The hRPTEC TERT1 cells (ATCC, CRL‐4031) were maintained in a DMEM/F12 medium (Cytivia), supplemented with 10 ng/mL of hEGF, 0.1 mg/mL of G418, 1% ITSG, 3.5 μg/mL of ascorbic acid, and 25 ng/mL of prostaglandin E1 (ATCC).

    Techniques: Gene Expression